Superoxide and hydroxyl radical, formed in biological
system, have been known to be highly reactive with a
wide range of molecules found in living cells (Valko et al.,
2007; Halliwell and Gutteridge, 2007). The EC50 values of
HWEP on superoxide and hydroxyl radical scavenging
activity were 446.3±12.1 and 706.3±16.3 µg/ml,
respectively. However, superoxide and hydroxyl radical
scavenging activity were twenty-fold and five-fold less,
respectively, than gallic acid. In biochemical systems,
hydrogen peroxide dismutates from superoxide radical,
can be converted to hydroxyl radical in the presence of
specific transition metal ions such as iron and copper
(Halliwell and Gutteridge, 2007; Wang et al., 2008). The
metal chelating activity of P. indica HWEP interfered with
the ferrous-ferrozine complex and varied inversely with
extract concentration. Nevertheless, the ferrous ions
chelating activity of HWEP was found to be very low
relative to the positive control EDTA. Additionally, HWEP
reduced Fe3+ to Fe2+ (Table 1).
Superoxide and hydroxyl radical, formed in biologicalsystem, have been known to be highly reactive with awide range of molecules found in living cells (Valko et al.,2007; Halliwell and Gutteridge, 2007). The EC50 values ofHWEP on superoxide and hydroxyl radical scavengingactivity were 446.3±12.1 and 706.3±16.3 µg/ml,respectively. However, superoxide and hydroxyl radicalscavenging activity were twenty-fold and five-fold less,respectively, than gallic acid. In biochemical systems,hydrogen peroxide dismutates from superoxide radical,can be converted to hydroxyl radical in the presence ofspecific transition metal ions such as iron and copper(Halliwell and Gutteridge, 2007; Wang et al., 2008). Themetal chelating activity of P. indica HWEP interfered withthe ferrous-ferrozine complex and varied inversely withextract concentration. Nevertheless, the ferrous ionschelating activity of HWEP was found to be very lowrelative to the positive control EDTA. Additionally, HWEPreduced Fe3+ to Fe2+ (Table 1).
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