Other authors also suggest that some anemonefishes,
particularly A. clarkii, are innately protected from sea
anemones and can produce their own protective mucous
coating (Miyagawa and Hidaka, 1980; Miyagawa, 1989). On
the other hand, by using enzyme-linked immunosorbent
assay (ELISA) Elliott et al. (1994) showed that anemone
mucus antigens were present in the mucous epidermal
layer of the associated anemonefish A. clarkii, which had
been in contact with the sea anemone, but those antigens
were absent in fish which had never been encountered sea
anemones.