The bacterial strains obtained from Institute of Microbial Technology, Chandigarh, were used for evaluating antimicrobial activity.
The bacterial and fungal stock cultures were incubated for 24 hours at 37°C on nutrient agar and potato dextrose agar (PDA) medium (Microcare laboratory, Surat, India), respectively, following refrigeration storage at 4°C. The bacterial strains were grown in Mueller-Hinton agar (MHA) plates at 37°C (the bacteria were grown in the nutrient broth at 37°C and maintained on nutrient agar slants at 4°C), whereas the yeasts and molds were grown in Sabouraud dextrose agar and PDA media, respectively, at 28°C. The stock cultures were maintained at 4°C.