2.2. Seed material
Wheat seeds (Triticum aestivum) were chosen for this study, since wheat represents one of the most important crop plants all over the world. The seedswere obtained froma private collection. Theywere visually scanned, and only healthy seeds without visible defects were selected
for treatment. Two seed lots were prepared, each lot containing 105 seeds. The seeds from one lot were exposed to plasma, while the seeds from the other lot were used as control. The seeds to be treated were distributed uniformly on the wires in such a way that individual seeds did not touch each other and exposed to plasma for a duration of 5, 15, 30 min.Plasma treated and control seeds were placed into Petri dishes (90 mm diameter) containing one layer of filter-paper impregnated with 5ml distilledwater. Each Petri dish contained 15 seeds. The dishes
were coveredwith lids andwrapped using a strip of parafilmto prevent evaporation of water. Wheat seeds were cultivated in an incubator at 22 °C, in the dark for four days.