because patient microbiota may colonize health care personnel and an ambulance's environment during their as-sistance. Contaminated hands are main sources of microbial transmission causing health care-associated infections . Basic life Support ambulances(BLSAs) link the community and health care facilities and a lack of basic infection control measures could promote the exchange of MDROs. This study aimed to analyze microbial contamination and antimicrobial resistance profiles of clinically rel- evant microorganisms isolated from BLSAs. A cross-sectional study was conducted during July 2012 in 10 of 17 RI SAC from the Bilbao,Spain,metropolitan area emergency