The second attempt of AI used alpaca semen collected by
electroejaculation (Calderón et al., 1968). Ninety-six female
alpacas were artificially inseminated at different intervals
from induction of ovulation. A 75% fertilization rate (fertilized
ova recovered 3 days after insemination) was obtained
when insemination was performed between 35 and 45 h of
induction of ovulation (Table 4; Calderón et al., 1968). A
third account in AI used 83 female alpacas and 11 female
llamas and vicuna and paco-vicuna semen was collected by
electroejaculation. Ovulation was induced by the administration
of hCG or copulation with a vasectomized male
(Leyva et al., 1979). Undiluted fresh semen was deposited
into the uterine horns via the cervix. The overall percentage
of pregnant females was 31%. For hCG-treated females,
pregnancy rate was 48% and 11% for vasectomized male
induced ovulation (Leyva et al., 1979). The pregnancies
generated by AI using frozen–thawed alpaca semen are
variable, 29–35% (7 crias out of 22 females inseminated) of
live crias born have been reported.