Healthy adult male and female Wistar rats,
Rattus norvegicus, 5-7 weeks old were used in
acute dermal toxicity test. They were supplied
by the National Laboratory Animal Center,
Mahidol University, Salaya, Nakhon pathom.
They were kept in controlled conditions at the
room temperature of 22-25°C and relative
humidity of 65 ±5 percent. They were housed in
stainless steel cages, 5 animals with the same
sex per cage and were fed with food (Pokphan
Animal Feed Co., Ltd., Thailand) and water ad
libitum