Yeast-extract peptone (YP) media was prepared by dissolving
1 g yeast extract (Y-0500, Sigma) and 2 g peptone
(Cat #: P-6713, Sigma) in 100 mL deionized water. The
YP media was sterilized by autoclaving for 15 min at
121 C. The YPD medium was then prepared from the YP
medium by aseptically adding 5 g glucose (G-7528,
Sigma) before storage at 4 C.