Significant increase in artemisinin content in various plant organs was found in all the treatments (Tables 5–7). Artemisinin has been reported to accumulate in leaves, 89% of the total artemisinin of the plant is found in leaves (Ferreira and Janick, 1995). Therefore, substances that improve the leaf development and shoot growth are presumed to increase artemisinin yield. Moreover, biosynthesis of terpenoid is dependent on primary metabolism, e.g. photosynthesis and oxidative pathways for carbon and energy relationship between primary and secondary metabolism, leading to increased biosynthesis of secondary products supply (Singh et al., 1990). Factors that increase dry mater production may influence the inter (Kapoor et al., 2007). van Gelgre et al. (1997) suggested that significant improvement in plant biomass may result in greater availability of substrate for artemisinin biosynthesis. The enhanced concentration of artemisinin by organic manure and chemical fertilizers may be due to improved growth and nutrient status of the plants. The maximum content was found in plants receiving nitrogen and sulphur in two equal split along with basal dose of phosphorus and potassium (T4). It is well documented that approximately 60% of nitrogen in plant leaf is invested in photosynthetic components. Thus, nitrogen nutrition plays a crucial role in determining the photosynthetic capacity of plant in both natural and agricultural environment (Kumar et al., 2003). While nitrogen directly affects the photosynthesis efficiency of the plant, sulphur affects the photosynthesis efficiency indirectly by improving the nitrogen utilization efficiency of the plants as shown by Ahmad and Abdin (2000a). Consequently, adequate sulphur supply increases the nitrogen utilization into protein (Rubisco) synthesis, thereby enhancing the photosynthetic rate.