Effect of inoculums size on biosurfactant production
To optimize the amount of inoculums for biosurfactant production, we varied the concentration of inoculums from 2.0 to 12.0 % (v/v). AS shown in Table 2, with an increase in inoculums size from 2.0 to 8.0 %, growth and biosufactant production by D. caeni PO5 was enhanced linearly. Although the dry cell weight seemed to increase with an increase in the amount of inoculums (2.0-12.0 %), there was no corresponding increase in the amounts of excreted biosufactants.