2.7. Parasite burden
Fish were anaesthetised in etomidate (2 mg/L) and as a standard,
parasites on the caudal fin, the anal fin, the left pectoral and
the left pelvic fin were counted using a dissection microscope. The
parasite burden was estimated from 5 fish, 4 days following the first
infection and it was estimated from 5 naive tra; nac, 6 naive WT
zebrafish and all 8 survivor fish two days after the secondary
infection.