Autoclave at 121 องศาเซลเซียส นาน 15 นาที . Medium pH should be 6.8 บวกลบ 0.2 after sterilization. After sterilization, immediately place tubes in an inclined position so the agar will solidify with a sloped surface.
Autoclave at 121 ° C for 15 minutes. After sterilization, the medium pH value should be 6.8 plus or minus 0.2. Immediately after sterilization Place the pipe in an inclined position so that Agar can use a slow surface reinforcement.