Chitosan samples dried in the oven
at 90 1C were solubilized in acetic acid and submitted to titration
with 0.1 M NaOH , yielding a curve with two inflection points.
The difference in NaOH volume used between the two inflection
points corresponds to the acid consumed for the salification of the
amino groups of chitosan, and the degree of acetylation was
calculated by