Microsatellites
Individual genotypes were obtained by cross-species amplification of four
microsatellite loci (LCO1, LCO3, LCO4, and LCO5) and genome-specific allele
identification, as already used in the study of the complex [26]. Diploid
S. alburnoides were genotyped as PA (hybrids) and AA (nuclear ‘‘nonhybrid’’).
All triploids were genotyped as PAA from fin clips and identified by
the presence of three alleles for at least one locus. Organ genotypes in
each individual were checked by parallel amplification of the four microsatellite
loci.