Methods
1. Sugar after fermentation volume of 1 ml was put in a test tube
2. 9 ml of distilled water in a test tube containing the sample
3. Take a test tube containing sugar after fermentation and distillation into a blender mix. To make the mixture into
four. Using suction pipette solution to it. In vitro, 1 ml volume
of 2 ml 5. Add distilled water in a test tube in Article 4
6. Remove the tube with a solution that has been put in a blender mix. To make the mixture into
seventh. Using suction pipette solution to it. In vitro, two tubes of each tube and 0.5 ml of distilled water in a test tube volume of 0.5 ml
test tube 8. Remove the 3 tube into DNS three doses 0.5 ml tube
9. All three trials led to a spin in the blender, mix
10. Take a test tube with a solution of three tubes to boil 15 minutes using a ball gag bottle
11. After 15 minutes, let it cool down to room
12. 4 ml of distilled water three tubes were then spun in a blender, mix
13. To add to measure the absorbance
14. Take the test results were analyzed.