Three mycelial disks (5 mm in diameter) from 10-d-old
cultures were inoculated into conical flasks (250 mL)
containing 100 mL of potato dextrose broth (PDB) (Oxoid)
with or without 1% chitosan (pH 5.0) at 180 r min–1 at 25°C.
Fungal biomass was collected, washed thoroughly three
times with sterile double-distilled water, and then placed
on filter paper for 2 h. The fresh weight was assayed each
day for 5 d.