II. MATERIALS AND METHODS
2.1 Plant Materials
Fresh leaf of Dendrophthoe pentandra (L.) Miq was collected from Curup, Bengkulu Province, Indonesia. Taxonomic authentication of the sample was conducted by a botanist in the Herbarium Bogoriense, Research Centre for Biology-Indonesia Institut of Sciences, Bogor, Indonesia.
2.2 Extraction and Fractination
Extractions were conducted by boiling and maceration. Comparison of the clove mistletoe leaf powder with solvent is 1:10. Boiling was conducted by boil the leaf powder into the water solvent for 2 hours while maceration by it immersing into ethanol 70 % for 24 hours with a shaker in speed of 150 rpm. The mixture was separated and evaporated using an evaporator to obtain water and ethanol extracts. The ethanol extract further fractionated with a solvent which has a different polarity, i.e. n-hexane, ethyl acetate and ethanol. The test samples in this study consist of water and ethanol extracts as well as n-hexane, ethyl acetate and ethanol fractions.