Shaking flasks experiment
Precultures were prepared from solid medium and incu-
bated for 24 h at 35°C and 120 rpm. When the precul-
tures reached an OD420 of 132, pH value 67, 10 ml was
used to inoculate two parallel set-ups with 250 ml of
modified M medium in 1-litre flask. Glucose was added
as a concentrated solution (50% w/v) during fermenta-
tion to avoid the carbon source limitation. Growth was
monitored via optical density at k of 420 nm.