2.5. Selection of solvents for extraction of compounds from
urine
Initially, various organic solvents, namely, benzene,
chloroform, cyclohexane, dichloromethane (DCM), and
petroleum ether (40 C–60 C), were tested for extraction of
maximum number of compounds from buffalo urine. The
solvent-extracted materials were subjected to thin-layer
chromatography as per the methodology of Barman et al.
[19] after reconstitution in 1 mL solvent. DCM was finally
selected for the extraction of urinary compounds, on the
basis of the observations that it produced more number of
spots on thin-layer chromatography and was easier to
remove by evaporation compared with the rest of the solvents
due to its low boiling point (about 40 C).