Linearity was shown for acemannan between 100 and 800 ng/ spot by 4 different concentrations of the acemannan standard.
The equation y = 1.943 x + 7.103 (R2 = 0.999) with the applied acemannanmass x and the area y was obtained (l" Fig. 2). 356mg/g of acemannan was determined in the gel powder.