The culture medium for the runs was the final textile effluent itself, which was used after dilution according to a Factorial Design.
Algal cells were inoculated at a concentration of 20% (Vinoculation/Vmedia) in 500 ml Erlenmeyer flasks incubated at a thermo-statically controlled environmental chamber at 25± 1 C with a luminance of 3.000 lux and with a 12 hours
light/dark photoperiod (Tanticharoen et al., 1994) for 15 days.
The experiment was carried out in triplicate and average values were recorded.