Under aseptic conditions, sterilized AM film discs of 5 mm
diameter were placed on the agar surface inoculated with 200 ml of
microbial suspension (108 cfu/ml) and the antibacterial activity of
CH film with and without GEO was evaluated by disc diffusion
method using Mueller-Hinton agar (MHA) plates. Plastic film disc of
multilayer ethylene vinyl alcohol (EVOH) (nylon, EVOH and polyethylene)
was used as control. The plates were incubated at 37 C
for 18 h and the inhibition zones (mm) were measured with a scale
calliper. Experiments were performed in triplicate and mean value
was calculated