The method is selective for the determination of amoxicillin in the presence of its degradation products, other antibiotics and different amines that are normally encountered in dosage forms31. Amoxicillin has also been quantified in drug substances or formulations by polarography of a 6.2 pH solution, an acid hydrolysis or a bromine oxidation product. A
product formed by alkaline hydrolysis, heating with formaldehyde at pH 5 can be detected by a method based on polarography with limit of 10μg/ml for amoxicillin in plasma25. A Flow injection method has been used in which amoxicillin hydrolysed by immobilised β‐
lactamase, the penicilloic acid reacted with iodine and the 4blue starch‐iodine colour measured25. The quenching of fluorescence of mercurochrome has been used to assay amoxicillin & this measurement was made at pH 10 (Florescence method). A spectrofluorometric method based on degradation to a fluorescent product, measured after extraction into an organic solvent can be used which is capable of assaying amoxicillin in plasma or urine down to about 1μg/ml25. Nagaralli et al., 2002 reported a sensitive
spectrophotometric method for amoxicillin based on the measurement of absorbances of tris(o‐phenanthroline) iron(II) [method A] and tris (bipyridyl) iron(II) [method B] complexes at 510 and at 522 nm, respectively32. Microbiological assay by agar plate diffusion method with a sensitive strain of organism (Sarcina lutea or Bacillus sutilis) has been reported to assay amoxicillin in biofluids. However it is lengthy and not very sensitive method