In order to approach the problem with nitrogen limitation in high gravity beer fermentation and make nitrogen sources already present in the wort available to brewer’s yeast, we investigated the effects of proteases (Neutrase, Flavorzyme and Protamex) supple- mentation to high gravity worts (20 ◦ P and 24 ◦ P) on the FAN levels and fermentation performance of brewer’s yeast.