been used to measure total phenolics in natural matrices but since
its mechanism is an oxidation/reduction reaction, it may also be
considered as an antioxidant method (Prior et al., 2005). A skin
extract of regular orange carrot (Daucus carota L. ssp. sativus)
grown in the same location was also tested aiming to make a
comparison with the black carrot extracts. In order to avoid the
influence of carotenoids in black carrots analysis, extracts obtained
without the liquid–liquid extraction steps with chloroform,
pentane and cyclohexane were also analysed and the differences
observed between the relative amounts of the two carrot extracts
were similar (data not shown). The levels of phenolic compounds