In view of the poten tialuse of pyrolysis based technologies,it is crucialt ounder stand the environmental
hazards of pyrolysis-derived products,inpart icularbio-oils.Here,threebio-oil swere produced from fast
pyrolysis of pinewood and in ter mediate pyrolysis of corns talk and poultry litter.They were fully
characterizedby chemicalanalysis and tested for the irbiodegradabilityandtheirecotoxicityonthe
crustacean Daphnia magna and thegreenalga Raphidocelis subcapitata. Thesetestswerechosenasre-
quiredbytheEuropeanREACHregulation.Thesethreebio-oilswerebiodegradable,with40–60% of
biodegradation after28days,andhadEC50valuesabove100mgL1 for thecrustaceanandabove
10mgL1 for thealga,showinglowtoxicitytotheaquaticlife.Thetoxicunitapproachwasappliedto
verify whethertheobservedtoxicitycouldbepredictedfromthedataavailableforthesubstancesde-
tectedinthebio-oils.Thepredictedvalueslargelyunderestimatedtheexperimentalvalues.