This glass
plate was put inversely on a glass Petri dish (150 mm
diameter), aiming at to collect the eggs laid. The
engorged females were maintained in a Biological
Oxygen Demand incubator at 27 1 8C, RH 80%
and a 12:12 h photoperiod (Fig. 1A). In order to obtain
larvae of the same age cohort, egg batches were
collected daily in separate Hatchings tubes of polyethylene
with screw caps (Fig. 1B and C).