1. Is it true that eukaryotic chromosomes have multiple origins of replication?
2. Nucleotides are always added to the growing DNA strand at the 3’ end, at which the DNA has a free______ on the 3’ carbon of its terminal deoxyribose.
3. The E. coil chromosome has 4.7 * 106 bp; a bi-directional replication fork progresses at about 1000 nucleotides/sec. Therefore, the minimum time required to complete replication is……….min.
4. What is the sequence (1 to 6) in which these proteins function during DNA replication
____ RNA primase
____ DNA ligase
____ DNA polymerase
____ Topoismerase
____ DAN helicase
____ tethering proteins
5. Why is an RNA primer necessary for DNA replication?