with 40 mL ethanol/petioleum ether (1: 1 v/v) with magnetic stirring in a 70 °C water bath for 10 min. Afterwards, the resulting mixtmes wele centiifuged at 1800 x g for 5 min at 25 °C The p0“ der residue troni the bottom of the centrifuge tube was translei red to a 100-1111; beaker and subsequently dried at 70 °C for
approximately 24 h until constant Weight. The total oil content was expressed as follows:
Total oil con tent =
The beaker containing barrier residue --~ The clean beaker
The weight of 111icrocapsules
Encapsulation efficiency The encapsulation etticiencva iough measure of the pe1cent of
the axailable oil that IS no longer easily 1emoved, was calculated using the following equation (Bae and Lee 2008):
, -, Total oil -Easily remo red oil _ Encapsulation ett1c1ency = -------------;------~---x 100 Total 011
Encapsulation yield
The encapsulation yield was defined as the ratio (expressed as a percentage) of total fish oil in the microcapsules compared to the fl$h oil in the emulsion prior to spray drying (Young and others
1993).