and the mixture was left for 24 h at room temperature, neutralized to pH 6.0 with acetic acid (50 mL/100 mL), dialyzed as described above, and re-concentrated to 10 mL
and the mixture was left for 24 h at room temperature, neutralized to pH 6.0 with acetic acid (50 mL/100 mL), dialyzed as described above, and re-concentrated to 10 mL