SDS-PAGE
The molecular weight distribution of the sample was determined
using SDS-PAGE according to the method of Laemmli [35].
The sample was mixed with the sample buffer (0.125M Tris–HCl,
pH 6.8, containing 4% (w/v) SDS and 20% (v/v) glycerol) with and
without ˇME. The proteins (4g) were loaded into the polyacrylamide
gel (15% running and 4% stacking gels) and subjected to