Interestinthermophilicbacteriaaslive-cellcatalystsinbiofuelandbiochemicalindustryhassurgedinrecent
years, duetotheirtoleranceofhightemperatureandwidespectrumofcarbon-sourcesthatincludecellulose.
Howevertheirdirectemploymentasmicrobialcellularfactoriesinthehighlydemandingindustrialconditions
has beenhinderedbyuncompetitivebiofuelproductivity,relativelylowtolerancetosolventandosmicstresses,
and limitationingenomeengineeringtools.Inthisworkwereviewrecentadvancesindissectingandengineering
the metabolicandregulatorynetworksofthermophilicbacteriaforimprovingthetraitsofkeyinterestinbiofuel
industry:cellulosedegradation,pentose–hexoseco-utilization,andtoleranceofthermal,osmotic,andsolvent
stresses.Moreover,newtechnologiesenablingmoreefficientgeneticengineeringofthermophileswerediscussed,
suchasimprovedelectroporation,ultrasound-mediatedDNAdelivery,aswellasthermo-stableplasmidsandfunc-
tionalselectionsystems.Expandedapplicationsofsuchtechnologicaladvancementsinthermophilicmicrobes
promisetosubstantiateasyntheticbiologyperspective,wherefunctionalparts,module,chassis,cellsandconsortia
weremodularlydesignedandrationallyassembledforthemanymissionsatindustryandnaturethatdemandthe
extraordinarytalentsoftheseextremophiles.
Interestinthermophilicbacteriaaslive-cellcatalystsinbiofuelandbiochemicalindustryhassurgedinrecent
years, duetotheirtoleranceofhightemperatureandwidespectrumofcarbon-sourcesthatincludecellulose.
Howevertheirdirectemploymentasmicrobialcellularfactoriesinthehighlydemandingindustrialconditions
has beenhinderedbyuncompetitivebiofuelproductivity, relativelylowtolerancetosolventandosmicstresses,
และ limitationingenomeengineeringtools.Inthisworkwereviewrecentadvancesindissectingandengineering
metabolicandregulatorynetworksofthermophilicbacteriaforimprovingthetraitsofkeyinterestinbiofuel
industry:cellulosedegradation,pentose–hexoseco-utilization,andtoleranceofthermal,osmotic,andsolvent
stresses.Moreover,newtechnologiesenablingmoreefficientgeneticengineeringofthermophileswerediscussed,
suchasimprovedelectroporation,ultrasound-mediatedDNAdelivery,aswellasthermo-stableplasmidsandfunc-
tionalselectionsystems.Expandedapplicationsofsuchtechnologicaladvancementsinthermophilicmicrobes
promisetosubstantiateasyntheticbiologyperspective,wherefunctionalparts,module,chassis,cellsandconsortia
weremodularlydesignedandrationallyassembledforthemanymissionsatindustryandnaturethatdemandthe
extraordinarytalentsoftheseextremophiles.
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interestinthermophilicbacteriaaslive cellcatalystsinbiofuelandbiochemicalindustryhassurgedinrecentปี duetotheirtoleranceofhightemperatureandwidespectrumofcarbon sourcesthatincludecellulose .howevertheirdirectemploymentasmicrobialcellularfactoriesinthehighlydemandingindustrialconditionsมี beenhinderedbyuncompetitivebiofuelproductivity relativelylowtolerancetosolventandosmicstresses , ,limitationingenomeengineeringtools inthisworkwereviewrecentadvancesindissectingandengineering และ .การ metabolicandregulatorynetworksofthermophilicbacteriaforimprovingthetraitsofkeyinterestinbiofuelอุตสาหกรรม : cellulosedegradation นาคร ) , hexoseco ใช้ andtoleranceofthermal การ andsolvent , , ,ความเครียด นอกจากนี้ newtechnologiesenablingmoreefficientgeneticengineeringofthermophileswerediscussed , ,suchasimprovedelectroporation , อัลตราซาวนด์ mediateddnadelivery aswellasthermo stableplasmidsandfunc - ,expandedapplicationsofsuchtechnologicaladvancementsinthermophilicmicrobes tionalselectionsystems .promisetosubstantiateasyntheticbiologyperspective wherefunctionalparts , แชสซี , cellsandconsortia โมดูลweremodularlydesignedandrationallyassembledforthemanymissionsatindustryandnaturethatdemandtheextraordinarytalentsoftheseextremophiles .
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