The solid medium containing 100 g of dry substrate was dispensed into an Erlenmeyer flask of 250 mL capacity. The flasks were sealed with air filter membranes, autoclaved twice for 30 min
each at 121 ºC. After sterilization, each flask was surface inoculated with mycelia from PDA medium (three discs, 10 mm in diameter). Three replicates were prepared for each treatment, and an uninoculated
flask served as control. All the flasks were incubated at 25 ºC in the dark. The entire contents of flasks were harvested at 5, 10, 15, 20 and 30 days after the mycelia extend into the whole
medium. Then the samples were dried to constant weight at 60 ºC and used for determining the main nutritional components and antioxidant properties