The total phenolic content of the extract was measured with the
Folin–Ciocalteau method as described by Taga, Miller, and Pratt
(1984), using gallic acid as a standard. A 0.1 ml of the extract solution (1.0 mg/ml DMSO) was sampled into 2 ml of 2% Na2
CO3 and
mixed for 3 min. After adding 0.1 ml of 50% Folin–Ciocalteau reagent, the final mixture was left for 30 min before reading the
absorbance at 750 nm (Hitachi U-2000, Tokyo, Japan). All measurements were conducted in triplicate and the data were expressed as
g gallic acid equivalent (GAE) per kg of the extract, based on the
calibration curve of gallic acid.
The total phenolic content of the extract was measured with theFolin–Ciocalteau method as described by Taga, Miller, and Pratt(1984), using gallic acid as a standard. A 0.1 ml of the extract solution (1.0 mg/ml DMSO) was sampled into 2 ml of 2% Na2CO3 andmixed for 3 min. After adding 0.1 ml of 50% Folin–Ciocalteau reagent, the final mixture was left for 30 min before reading theabsorbance at 750 nm (Hitachi U-2000, Tokyo, Japan). All measurements were conducted in triplicate and the data were expressed asg gallic acid equivalent (GAE) per kg of the extract, based on thecalibration curve of gallic acid.
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