using the Least Significant Difference (LSD) at P < 0.05. Spectra were exported from
the OPUS software into The Unscrambler X software (version X, CAMO ASA,
Oslo, Norway) for chemometric analysis, data processed using the second
derivative Savitzky–Golay and then Principal Component Analysis (PCA)
was used to interpret the changes in the MIR spectra of the samples related to
storage, as per Cozzolino et al. (2013).