10 L of sample was added to acetone cleaned 12 mm Micro cover glass slides and allowed to adhere 60 min in a 6 well petite dish at room temperature. then 100 ul of 2.5% gluteraldehyde, was added and allow to fix for 30 min. the samples were then rinsed twice for 3o min each with approximately 2-3 ml of the 0.1 M imidazole,followed by 30 min intervals each 50 80 90% ethanol 2-3 ml each