2.4.5. Superoxide anion radical scavenging activity
The superoxide anion radical scavenging activity of the sample was investigated by the method of .In this estimation, 50 ll of NADH (468 lM), 50 ll NBT (150 lM) and 50 ll phenazine methasulphate (PMS, 60 lM) were added to the various concentrations of the samples. All the above reagents were diluted in PBS (pH 7.4). The mixtures were incubated for 15 min in the dark and the absorbance measured at 560 nm. Trolox was used as standard and catechin was used as positive control. The superoxide anion radical scavenging capacity was calculated using the following equation: Superoxide anions scavenging activity % ผ ฝ๐A0 _ A1=A0_ _ 100; where A0 is the absorbance of the control (dH2O), and A1 is the absorbance of the extract/standard.