The current techniques available in literature related to methods
to quantify papain activity are in a short number and often linked
to intensive laboratory work, long periods of time, also involving
high cost equipment, reagents and substrates. As examples
researchers have shown the determination of papain activity using
synthetic substrates, e.g. benzyloxcarbonyl-phenylalanylarginine
7-amido-4-methylcoumarin [6] and N-Benzoyl-DL-arginine-ρ-
nitroanilide [5] based on the papain catalyzed conversion of the
compound