When bacteria and yeasts were both inoculated in the
vials and regardless of the bacterial concentration, no yeast
was observed on the Gram-stained aliquot of blood.
However, the presence of yeasts was quite easily detected
when only yeasts were inoculated in the bottles.
Moreover, no fungal growth has been detected when the
blood from the positive vials was plated on Mueller–Hinton
agar medium. Only bacteria were present on this medium.
For the couple C. albicans–E coli, the positive vials were
detected at a mean time of 11 h 2 min, regardless of the
bacterial concentration, whereas the vials inoculated with
C. albicans alone showed a detection time of 19 h 32 min.
Whatever the bacterial concentration, the culture on
Sabouraud–gentamicin medium of the blood samples
recovered from the vials did not show any fungal growth
for 3 vials/30 for the lowest bacterial concentration and for 5
vials/30 for the highest or medium bacterial concentration
(Fig. 1).
For the couple C. albicans–S. aureus, the positive vials
were detected at a mean time of 15 h 13 min, regardless of
the bacterial concentration. The number of vials in which it
was not possible to detect fungal growth was 4 vials/30 for
the lowest bacterial concentration and 5 vials/30 for the
medium concentration and increased to 10 vials/30 for the
highest concentration (Fig. 1).
Concerning C. glabrata, the detection time of vials
inoculated with this yeast alone was about 48 h 12 min. The
results obtained with the co-inoculation of C. glabrata–
bacteria showed that the growth of the yeasts was quite
inhibited by the bacterial development in the bottles. When