At metamorphic climax (stage G42: determined as forelimbs
emergence), the larvae were euthanized and then measured every
parameter (body mass, intestine mass, total body length, tail
length and hind-limb length). The exposure ended when half of larvae
in control groups completed metamorphic climax. Until the
end of experiment, each individual was weighted once on an analytical
balance having readability of nearest 0.001 g. Lengths of the
larvae were measured to the nearest 0.01 mmby Tesa-Cal Dura-Cal
Digital electronic calipers. In addition, the number of larvae initiating
forelimb emergence was also recorded daily for each replicate
aquarium.