The solution was evaporated with a vacuum evaporator at
40 C and then titrated three times with ethyl acetate (CH3COOC2H5),
using 50 ml each time. The upper transparent portion of the solution
mixed with sodium sulphate (Na2SO4) to absorb the remaining
water. Evaporation was again conducted until the solution was dry.
The residues thus obtained were true root exudates (Tang and
Young, 1982) and were stored at 20 C until further use.
From the 200 bulbs of each cultivar, 0.128 g of garlic root exudate
dry matter was collected from G064, and 0.09 g was collected from
G25. We then took an equal amount (0.09 g), measured using an
analytical balance, from each cultivar and dissolved them in
acetone; these solutions were mixed with 15 ml of autoclaved
distilled water, obtaining stock solutions (i.e., T4-100%). Three
concentrations (T1-25%, T2-50% and T3-75%) were prepared from
the stock solution of each cultivar by adding the calculated amount
of autoclaved distilled water. However, the stock solution was used
as treatment T4-100%.