A comprehensives survey was carried out in eight
different localities in Multan district and disease
incidence and loss assessment due to root infecting fungi
was calculated. Sampling of the diseased plants was done
by uprooting the whole plant from soil with the help of a
spade. Effort was made to take out the entire root system
by digging the roots carefully. After excising the aerial
portion and removing soil from the root system of the
uprooted plants, the roots were placed in polyethylene
bags. All the bags were tied and labeled. Three to four
samples were taken from a single site. Rhizosphere
soil/nodule samples were collected from the same site.