The main experiment was prepared as previous test, but inoculum (1 ml) was added to 300 ml flasks containing 100 mL of Kimura medium. On the basis of results of the previous test, dye concentration chosen for this part of experiment was 0.05 g l−1. Water solution of diazo dye Evans blue (Sigma-Aldrich) was filter-sterilized (Millipore cellulose filters ∅ 0.20 μm) and added to 48-h-old bacterial samples (stationary growth phase). Cultures were incubated in static, semi-static and shaken conditions in 26° C. Dead biomass (autoclaved for 20 min in 121 °C, 1.5 atm.) was used for estimation of biosorption.