Then neutralized with solid sodium carbonate until the effervescence ceases and then centrifuged. 0.1 ml of the hydrolyzed supernatant was pipette each into test tube and 0. ml of 5% phenol solution was added. Then, 0.5 ml of 96% sulphuric acid was added and vortexed thoroughly. The contents were allowed to stand for 30 min at room temperature. A volume of 200 ul from each test tube was transferred to 96-wells microplate. Absorbance was read at er nm using micro plate reader (UV/VIS-Spectrophotometer OPTIMA SP3000-PLUS). Blank sample was prepared using dristilled water. The total sugar content was calculated from the glucose standard.