Sampling of broodstock and production of family
lines have been described in detail by Kenway et al.
(2006). Briefly, male and female wild broodstock (G0)
were sampled from the north-eastern Australian coast.
Reproductive, weight and pedigree data were derived
from three generations (G1, G2 and G3). The G1 consisted of ten full-sib families, with three females
spawning twice and all other females spawning once
to yield 13 spawning events; the G2 comprised seven
paternal half-sib, three maternal half-sib and 19 full-sib
families, with one female spawning four times, ten females spawning three times, seven females spawning
twice and all other females spawning once to yield 65
spawning events; and the G3 comprised two paternal
half-sib family groups (with one sire mated to two dams
and the other sire mated to three dams) and 11 full-sib
families, with all females spawning once to yield 14
spawning events. Matings were achieved through artificial insemination with average inbreeding of female
broodstock being 0.0, 0.05 and 0.02 in generations G1,
G2 and G3 respectively.