Heavily boron doped diamond porous membranes were fabricated using low cost commercial fiberglass filters.Weoptimized the synthesis process to ensure pore-size control in a highly reproducible manner. They benefit from the diamond surface properties, namely the versatile surface chemistry. Thanks towell-mastered functionalization routes, diamond porousmembranes can exhibit tunable hydrophilic character or more advanced affinities. We have demonstrated that diamond membranes can be used as solid phase for analyte extraction in aqueous phase, using BSA model. Two characteristic peptides of BSA have been targeted and detected through mass spectrometry. The detection performances were improved with a factor of twenty thanks to a C4 functionalization. Via a convenient, simple and fast grafting technique, themajor effect of the surface state towardmembrane extraction capacity has been then pointed out. Forthcoming work will also assess other functionalization moieties and extraction efficiency toward a batch of usual proteins. Further, a preliminary electrochemical study has demonstrated that HBDD membranes exhibit large surface area that could potentially be highly attractive for catalysis, enzymatic reactor or electrochemical capacitor uses.