Determination of total soluble sugar and reducing sugar contents
Frozen broccoli florets (5 g) were extracted in 5 mL distilled
water and incubated at 85 C for 30 min, then centrifuged at
10,000 g at 4 C. Reducing sugar content was assayed spectrophotometrically
at 540 nm with 3,5-dinitrosalicylic acid by Wang
(2005). Total soluble sugar content was determined using anthrone
reagent and glucose as standard.