In preliminary test, protoplast could not be released from mycelia incubated at 30C for 1 hr in mycolytic enzyme solution that contained 0.1 M ac etate buffer (pH 5.0), 5% Funcelase and 0.6 M NaCl. This result indicated that mycolytic enzymes includ ing 1,3 -glucanase, chitinase and protease in the solution must be well balanced qualitatively and quantitatively.