Microorganisms were acquired from the Universidad de las Americas Puebla collection. E. coli (ATCC 25922) and S. typhimurium were grown in nutritive broth at 37 °C until they reached the early stationary phase (14 h approximately). Five milliliters of inoculum [(2.60 ± 0.21) × 107 CFU/mL)] were added to 100 mL of coconut milk to reach an initial microbial load of about 5–6 log cycles