In contrast, the quantification method using an internal standard
material does not depend on a model-matrix. However, the
coefficient value needs to be determined for each batch of internal
standard material. In addition, high standard deviations of the
determined sesame contents were observed. The quantitative results
are calculated from initial target sequence copy numbers
determined using two independent real-time PCR systems. Therefore,
the variability of the copy numbers of both real-time PCR systems
contributes to the variation of the quantitative results.